Illinois Department of Agriculture - Animal Disease Laboratories
Centralia - Galesburg - Springfield - University of Illinois, VDL
Laboratory User's Manual
 


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Consideration

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Mycology

 
1.
 For dermatophyte cultures, the affected area of skin should be washed with 70% alcohol, scrapings taken from the active border areas and placed in a sterile container. The basal portion of several hairs should be plucked out with forceps and submitted as well.

2.

 Exudates or tissues for culture should be collected aseptically, refrigerated, and sent to the laboratory on ice packs. Hair and skin scrapings for dermatophyte isolation should be shipped dry at room temperature in a paper envelope.
3. Isolation and identification of some mycotic agents may require a minimum of 30 days.
4.

We will routinely culture and identify samples from suspected aspergillosis, candidiasis, cryptococcosis, dermatomycosis, mucormycosis, mycotic abortion, and sporotrichosis.

Please inform us if blastomycosis, coccidiomycosis, or histoplasmosis are suspected so that proper precautions can be taken in the laboratory.

It is strongly recommended that tissues be submitted for histopathology rather than mycology in the initial evaluation of these cases. Call the laboratory for assistance if culture for these organisms is needed.